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Saturday, August 1, 2020 | History

2 edition of Immunohistochemistry in the study of normal and osteoarthritic articular cartilage found in the catalog.

Immunohistochemistry in the study of normal and osteoarthritic articular cartilage

Keld Ostergaard

Immunohistochemistry in the study of normal and osteoarthritic articular cartilage

by Keld Ostergaard

  • 247 Want to read
  • 12 Currently reading

Published by Gustav Fischer in Jena .
Written in English

    Subjects:
  • Osteoarthritis.,
  • Articular cartilage -- Diseases.

  • Edition Notes

    Includes bibliographical references (p. 146-165) and index.

    StatementKeld Ostergaard, Donald M. Salter.
    SeriesProgress in histochemistry and cytochemistry -- v. 33, no. 2.
    ContributionsSalter, Donald M.
    The Physical Object
    Paginationviii, p. [93]-168 :
    Number of Pages168
    ID Numbers
    Open LibraryOL15569572M

    Immunohistochemical study of the BMPs and their extracellular antagonists in osteoarthritic human knee joint Abstract The osteophytes are bone spurs overgrowing the edge of the articular carti-lage during the course of osteoarthritis (OA). The cellular mechanism of their development and growth resembles the intramembranous and endochondral. To investigate the distribution of the bone morphogenic protein (BMP) antagonist chordin in normal and osteoarthritic cartilage and synovial membranes, and its regulation in chondrocytes and synovial fibroblasts by inflammatory and growth sation of chordin in tissues was undertaken by immunohistochemistry and gene regulation was determined by real time polymerase chain reaction.

    Cartilage procurement and processing: Normal articular cartilage was harvested from femo ral condyles and tibial plateaus of human tissue donors. Osteoarthritic cartilage was obtained from patients undergoing knee replacement surgery. All tissue samples were graded according to a modified Mankin scale with a score of normal and a.   Articular cartilage tissue. Articular cartilage was obtained under the ethics committee approval held by the Sheffield Musculoskeletal Biobank (STH, SMB).All patients provided written, informed consent before participation. Cartilage blocks were taken from waste tissue within each anatomic compartment of the knee (medial and lateral tibio-femoral and patello-femoral .

    Osteoarthritis (OA) is the most common form of arthritis and characterized by degeneration of the articular cartilage. Mitogen-inducible gene 6 (Mig-6) has been identified as a negative regulator of the epidermal growth factor receptor (EGFR). Cartilage-specific Mig-6 knockout (KO) mice display increased EGFR signaling, an anabolic buildup of the articular cartilage, and formation of chondro. expression is increased in late-stage OA cartilage compared with normal and early degenerative cartilage (8–10). Phenotypic modulation, with expression of collagens normally absent in adult articular cartilage or at atypical sites in OA cartilage, has been proposed (11). Once the cartilage is severely degraded, the chondrocyte is unable to.


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Immunohistochemistry in the study of normal and osteoarthritic articular cartilage by Keld Ostergaard Download PDF EPUB FB2

Progr. Histochem. Cytochem. Vol. No 2(). Immunohistochemistry in the Study of Normal and Osteoarthritic Articular Cartilage Keld Ostergaard Donald M. Salter With 12 Figures and 12 Tables GUSTAV FISCHER Jena Stuttgart Lubeck Ulm KELD OSTERGAARD (~), M.

D., Ph. Osteoarthritis Research Unit, Institute for Inflammation Research,RHIMA-Center Cited by: in osteoarthritic cartilage degeneration.

In this study, we analysed the exact localization of type VI collagen in its relationship to the chondrocyte and the (inter)territorial cartilage matrix. Additionally, we were interested in its ultrastructural appearance in normal and osteoarthritic cartilage. Sections of human articular cartilage from macroscopically normal and osteoarthritic femoral heads were graded by the HHGS ( scale) twice by 3 observers, and intra- and interobserver.

Keld Ostergaard, Donald M. Salter, Immunohistochemistry in the Study of Normal and Osteoarthritic Articular Cartilage, Progress in Histochemistry and Cytochemistry, /S(98), 33, 2, (III), ().Cited by: The objective of this study was to assess whether macroscopically normal articular cartilage taken from joints containing focal osteoarthritic lesions is histologically similar to articular cartilage taken from macroscopically normal joints.

Metacarpophalangeal, proximal interphalangeal, and distal interphalangeal joints were obtained from 10 horses following euthanasia. Objective The purpose of this study was to identify genes that are differentially expressed in normal versus osteoarthritic human articular cartilage as either potential novel therapeutic targets or diagnostic markers of this disease.

Design mRNA was isolated from histologically normal and osteoarthritic adult human articular cartilage. The Differential Display technique was employed. (A and B) RT-qPCR. Human osteoarthritic cartilage and adjacent normal cartilage were collected and subjected to RT-qPCR analysis of miR *Pimmunohistochemistry.

Human osteoarthritic cartilage and adjacent normal cartilage were collected and subjected to the staining procedure (blue scale bar: µm). Current osteoarthritis (OA) histopathology assessment methods have difficulties in their utility for early disease, as well as their reproducibility and validity.

Our objective was to devise a more useful method to assess OA histopathology that would have wide application for clinical and experimental OA assessment and would become recognized as the standard method. 15 hours ago  Focal early osteoarthritis (OA) or degenerative lesions account for 60% of treated cartilage defects each year.

The current cell-based regenerative treatments have an increased failure rate for treating degenerative lesions compared to traumatic defects.

Mesenchymal stem cells (MSCs) are an alternative cell source for treating early OA defects, due to their greater chondrogenic potential. The histochemical and immunohistochemical analysis of cartilage tissue provide important information about many physiological and pathological processes in this tissue.

This chapter is designed to provide an overview of certain applications and techniques of histochemistry and immunohistochemistry for the study of cartilage.

Osteoarthritis (OA) is characterized by changes to the articular joint, including progressive degeneration of the articular cartilage. The pathogenesis of OA is complex, because it can result from multiple etiologies involving mechanical, genetic, traumatic, and metabolic factors 1, less of the cause, the changes that occur to the articular chondrocytes during OA, such as chondrocyte.

Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells.

Methods. Full thickness articular cartilage from grossly normal surfaces was analyzed from femoral heads, obtained at hip replacement surgery, from OA (n = 10) and fracture (n = 10) en, extracted by α-chymotrypsin, was characterized by SDS-PAGE/Western blot analysis, ELISA and immunohistochemistry using monoclonal antibodies specific to collagens types II and III.

OBJECTIVE The objective of this study was to detail the topographical and zonal distribution of α and β subunits of the integrin superfamily in normal and osteoarthritic cartilage. METHODS Immunohistochemistry utilising antibodies towards α and β subunits was performed on cryostat sections of human articular cartilage from macroscopically normal (n = 6) and osteoarthritic (n = 6).

Localization of Sulf-1 and Sulf-2 in normal cartilage. Representative sections of year-old (a, d) and year-old (g) normal cartilage (Mankin scores: 0 and 2) as seen on safranin O staining are shown (n = 8; 19 to 37 years old).Sulf-positive cells (brown staining) are present in the superficial zone and the top of the middle zone, and Sulf-2 expression is greater than Sulf-1 (b, c, e, f, h.

Articular chondrocytes were isolated from femoral condyles and tibia plateaus of postnatal day 5 mice. Cartilage tissues were digested with % collagenase type II, as previously described 6.

Cartilage explants were obtained from mouse knee joints and cultured in DMEM (Gibco‐BRL) containing 3′‐sialyllactose (, μM) with or without.

Osteoarthritis and Cartilage would like to invite contributions to a Special Issue on OA Prevention. The Special Issue will be Guest Edited by Ewa Roos, May-Arna Risberg and Christopher Little.

The submission deadline is Janu Full details and instructions for. To examine the activity of matrix metalloproteinases (MMPs) and aggrecanase in control and diseased human articular cartilage, metabolic fragments of aggrecan were detected with monospecific antipeptide antibodies. The distribution and quantity of MMP-generated aggrecan G1 fragments terminating in V.

OBJECTIVE—The objective of this study was to detail the topographical and zonal distribution of α and β subunits of the integrin superfamily in normal and osteoarthritic cartilage. METHODS—Immunohistochemistry utilising antibodies towards α and β subunits was performed on cryostat sections of human articular cartilage from macroscopically normal (n = 6) and osteoarthritic.

Changes in sulfation of cartilage glycosaminoglycans as mediated by sulfatases can regulate growth factor signaling. The aim of this study was to analyze expression patterns of recently identified extracellular sulfatases Sulf-1 and Sulf-2 in articular cartilage and chondrocytes.

Sulf-1 and Sulf-2 expressions in human articular cartilage from normal donors and patients with osteoarthritis (OA. CD98 in Normal and Osteoarthritic Chondrocytes l.

VOL. 3 NO. 3 Summer RESULTS Expression of CD98 in Human Articular Chondrocytes (Immunohistochemistry). Six-teen sections of normal articular cartilage were obtained from 4 men (age rangemean 55 years) and 7 women (age rangemean 75 years).

Normal articular carti.TEM study of normal articular cartilage showed scarce lipid droplets (Roy and Meachim, ). In an interesting study conducted by PGI Chandigarh and Christian Medical College Ludhiana groups.To further explore whether the chondroprotection of YGPs in osteoarthritic mice is through TGF-β signaling, Tgfbr2 was conditionally knocked out in the articular cartilage of mice at the age of 2 weeks in this study (Shen et al., ).

As expected in the present study, severe articular cartilage damage was also observed in TGF-βRII Col2ER mice.